Test of Antibacterial Potential of Honey from Apis mellifera Bees against Pseudomonas aeruginosa in Vitri

ANTIBACTERIAL POTENTIAL TEST OF HONEY FROM BEES Apis mellifera TO Pseudomonas aeruginosa IN VITRO
 
Background. The use of antibiotics by the public for the treatment of bacterial infections often causes various problems such as side effects, and the price is relatively expensive due to raw materials that are not yet available in the country. Honey is one of Indonesia's natural resources which is often used as a medicine for wounds (gangrene). Pseudomonas aerugenosa is one of the seven species of bacteria that are commonly found in infected wounds. The habit of the people who often use honey as a wound medicine raises the question of whether honey has the ability to inhibit the proliferation and kill the growth of bacteria in infected wounds. 

Destination : To determine the antibacterial potential of pure honey against
Pseudomonas aerugenosa in vitro.

Method : This research is an experimental study using Apis mellifera bee pure honey test material with three series of liquid dilution methods at concentrations of 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,5625%, 0,78125, 0,390625% and XNUMX%, one positive control was aquadest and bacterial suspension, one negative control was bacterial suspension with Ceftriazone. In this liquid dilution method, the minimum inhibition rate (MIC) and minimum killing rate (MBC) values ​​were obtained. Data were analyzed descriptively. 

Results : Research shows that Apis Mellifera honey has an antibacterial effect with a MIC value of 16,6% and a MIC value of 16,6%.

Conclusion : Pure honey has an antibacterial effect against P.aeruginosa
vitro

Keywords : P.aerugenosa-pure honey-KBM-liquid dilution method

Principal Advisor
Prof. Dr. Ngatidjan, M.Sc, Sp. FK (K)   

Companion Advisor  
dr. Siti Isti'anah