Antioxidant Power Test of Yellow Soybean Seed Aqueous Extract (Glycine max (L) Merill) compared to Vitamin E in CCI-Induced Male White Rats (Rattus norvegicus)

Abstract: The production of free radicals (oxidants) that are not in balance with antioxidant activity can cause cell damage. The need for antioxidants is absolutely necessary not only endogenous but also exogenous. Yellow soybean is one of the plants that has antioxidant activity, which can reduce the effects of free radicals while vitamin E is a powerful antioxidant which is used. This study aims to determine the antioxidant activity of water extract in the yellow soybean seed (Glycine max (L) Merill) compared to the decreased content of vitamin E in malondialdehyde (MDA) in the rat (Rattus norvegicus) male induced by CCL4. This study is an experimental research laboratory with a pretest-posttest control group. 25 male rats (Rattus norvegicus) were divided into 5 treatment groups. Group I (positive control vitamin E), group II, III, and IV were given water extract of yellow soybean (Glycine max (L) Meril) with a concentration of 2.5%, respectively, 10%, and 40%. Group V is a negative control distilled water. Treatment was given for 5 days. On the 6th day, all groups were treated with CCI4 0.2 ml/200 g BW. Antioxidant activity is measured by the decrease in serum MDA levels before and after treatment. Statistical analysis using One Way ANOVA. In One Way Anova test p value = 0.196 (p> 0.05), which showed no significant difference (not significant) toward the mean decrease in rat blood serum MDA in all groups. Water extract of yellow soybean (Glycine max (L) Merill) a difference between the antioxidant activity compared to vitamin E as an antioxidant in white rats (Rattus novergicus) male Wistar strain which has been induced CCL4 but this difference was not statistically significant.